高性能淀粉基酶固定化载体的制备研究  

Preparation of High-powered Cassava Starch-based Carrier for Immobilization of Enzyme

作　　者：罗发兴[1] 杨景峰[1] 罗志刚[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品科学》2008年第10期174-177,共4页Food Science

基　　金：广东省科技攻关项目(2005B20401006);广东省自然科学博士启动基金项目(5300170);广东省粤港招标项目(2005A20302003);华南理工大学自然科学基金项目(E5051040)

摘　　要：本实验采用生物酶法对淀粉进行处理,然后再用高碘酸钠对酶处理淀粉进行氧化,制备出了高性能的淀粉基酶固定化载体。对淀粉水解率、反应时间、温度、pH、高碘酸钠的浓度和高碘酸钠和淀粉的摩尔比等对载体性能的影响进行了研究。制备出的载体具有较强的吸附性能和大量的醛基,对酶具有较强的固定化能力。Starch was treated by a mixture of glucoamylase and amylase, and then was oxidized by sodium metaperiodate, to prepare high-powered strach-based carder for immobilization of enzyme. The effects of different parameters including degree of hydrolysis, reaction time, temperture, pH, concentration of sodium metaperiodate and molar ratio of sodium metaperiodate to starch on the capability of the cartier were studied. The carder has powerful capability of immobilizing of enzyme because of its powerful adsorption ability and a great amount of aldehyde groups.

关 键 词：淀粉 载体 酶固定化 

分 类 号：TS201.25[轻工技术与工程—食品科学]