酶法制取玉米水溶性膳食纤维的研究  

Study on Preparation of Water-soluble Dietary Fiber by Enzymlysis Method

作　　者：祝美云[1] 侯传伟[2] 魏书信[2] 陆丽利[1] 

机构地区：[1]河南农业大学食品科学技术学院,河南郑州450002 [2]河南省农业科学院,河南郑州450002

出　　处：《食品科学》2008年第10期211-214,共4页Food Science

基　　金：河南省科技厅重点科技攻关项目(072102110006)

摘　　要：采用纤维素酶和木聚糖酶对脱去淀粉和蛋白质的玉米皮进行处理,以得到水溶性膳食纤维。通过正交试验探究酶解过程的最佳工艺条件,从而提高不溶性膳食纤维的转化率。结果表明,在温度为45℃、pH值为4.8、料液比为1:12、酶解时间为8h、纤维素酶和木聚糖酶的添加量分别为1%和0.3%时,转化率最高,可以达到6.57%。其中,纤维素酶的添加量对试验影响显著性最大。Using cellulase and xylanase to degrate the corn bran which is treated by amylase and protease, water-soluble dietary fiber could be obtained. The orthogonal test was adopted to explore the best technological conditions of enzymolysis process, in order to enhance the conversion rate of water insoluble dietary fiber that the result indicated that the optimal enzymolysis conditions are as follows： temperature 45 ℃, pH 4.8, solid-liquid ratio 1：12, enzymolysis time 8 h, and addition amount of cellulase and xylanase 1% and 0.3 % respectively. The maximum conversion rate of water insoluble dietary fiber reaches 6.57%. The addition amount of cellulase has the most significant influence to the experimental result.

关 键 词：玉米皮 纤维素酶 木聚糖酶 可溶性膳食纤维 

分 类 号：TS239[轻工技术与工程—粮食、油脂及植物蛋白工程]