蒸发光散射检测器在叶黄素HPLC定量分析中的应用  

Application of Evaporative Light Scattering Detector in Iutein Quantification by HPLC

作　　者：蔡靳[1] 惠伯棣[1] 

机构地区：[1]北京联合大学应用文理学院,北京100083

出　　处：《食品科学》2008年第10期508-511,共4页Food Science

基　　金：北京市教育委员会科技发展计划面上项目(200411417003)

摘　　要：目的:评价叶黄素使用HPLC-ELSD进行定量分析的可能性。方法:在C30-HPLC上应用ELSD的条件:漂移管温度45℃,喷嘴温度40℃,增益1。以ELSD色谱图中的全反式叶黄素组分峰面积为标准,对ELSD的载气流量(以压力表示)进行优化。在相同色谱条件下,对叶黄素进样量与ELSD峰面积进行回归计算,确定二者的相关关系,并测定叶黄素的检测下限。结果:在最适载气压力(30psi)条件下,在10～100μg范围内,全反式叶黄素的进样量与ELSD峰面积之间在一定置信度上呈现线性关系。ELSD的检测下限为6.4111μg。结论:在一定的浓度范围内,按照线性关系,应用ELSD对叶黄素进行HPLC定量是可能的。Objective： To conduct the possibility assessment for lutein quantification by hight performance liquid chromatographyevaporative light scattering detector （HPLC-ELSD）. Method： ELSD conditions on C30-HPLC were as follows： drift temperature 45 ℃, sprayer temperature 40 ℃ and gain 1. According to the peak area of all E-lutein on ELSD chromatogram, carrier gas flow rate （represented with pressure） was optimized. Under fixed chromatographic conditions, a regression of injection amount with peak area by ELSD was assessed for lutein. Additionally, the detection limit was determined. Results： Under optimized carrier gas pressure （30 psi）, a linear correlationship between injection amount （in the range of 10- 100 ug） of all E-lutein and peak area by ELSD appears at a confidence level. Conclusion： It is possible for ELSD to be applied in lutein quantification by HPLC according to the linear relationship between peak area by ELSD and injection amount in a lutein amount range.

关 键 词：蒸发光散射检测器 叶黄素 

分 类 号：TS201.21[轻工技术与工程—食品科学]