ATP代谢物安培酶生物传感器的研制及在鱼肉鲜度测定中的应用  

Preparation of Amperometric Enzyme Biosensors for ATP Metabolites and the Application for Detecting Fish Freshness

作　　者：干宁[1] 王鲁雁[1] 李天华[1] 陈晓东[2] 徐伟民[1] 

机构地区：[1]新型功能材料及其制备科学国家重点实验室宁波大学材料科学与化工学院,浙江宁波315211 [2]宁波大学生命学院,浙江宁波315211

出　　处：《中国食品学报》2008年第6期48-52,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省自然科学基金项目(Y4080023);宁波市自然科学基金项目(No.2008A610048;2008A610072);宁波市4321人才工程资助项目

摘　　要：目的:研制一种通过测定鱼组织中三磷酸腺苷(ATP)降解产物次黄嘌呤(HX)、肌苷(INO)、肌苷酸(IMP)代谢物来定量检测鱼肉鲜度的生物传感器系统。方法:用K1或H表示鲜度,K1或H取决于鱼肉中ATP降解产物的量。降解产物通过安培法检测,利用3种以二茂铁甲酸(FCA)为媒介体的导电聚吡咯酶电极,分别固定黄嘌呤氧化酶(XO)、核苷磷酸化酶(NP)和核苷酸酶(NT),定量检测HX、INO和IMP。结果:该传感系统可以快速测定HX、INO、IMP含量。同时发现即使鱼肉被储存于-12℃,7d后也会迅速分解。结论:该传感器简单、快速、使用方便,用来检测淡水鱼的鲜度,效果令人满意。Object： To develop a biosensor system for quantitative measurement of fish freshness by determining the ATP degradation products, hypoxanthine（HX）, inosine（INO）and inosine monophosphate（IMP）metabolites in fish- tissue. Methods： Freshness is indicated by an index K1 or H which depends on the amount of these ATP degradation products present in fish meat. These degradation metabolites have been detected efficiently by amperometric method using three new ferrocene carboxylic acid（FCA） mediator incorporated conducting polypyrrole enzyme electrodes with immobilized xan-hine oxidase, nucleoside phosphorylase and nucleotidase enzymes for quantitative measurement of HX, INO and IMP respectively. Results： The biosensor can be employed for determination of fish freshness.The fish degrades very rapidly after 7 days even when stored at -12℃. Conclusions： This simple, fast and ready to use biosensor has been used to measure the H values of fresh water over days of storage.

关 键 词：鱼肉鲜度 安培生物传感器 酶电极 次黄嘌呤 肌苷 肌苷酸 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]