流动注射催化光度法测定水产品中的甲醛含量  

Determination of Formaldehyde in Aquatic Products by Flow Injection (FI) Coupled with Catalytic Spectrophotometry

作　　者：陈婷[1] 黄志勇[1] 戴玉雷[1] 李连平[1] 

机构地区：[1]集美大学生物工程学院,厦门361021

出　　处：《中国食品学报》2008年第6期149-154,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金资助项目(40771185);福建省科技计划重点项目(2007Y0028)

摘　　要：目的:利用甲醛在磷酸介质中催化溴酸钾氧化结晶紫的反应,结合流动注射技术测定水产品中甲醛的含量。方法:通过一自制的三通装置,借助蠕动泵将流路中产生的气泡在线抽排出去,使催化反应能够在较高的温度下进行,并使管路溶液不断流,从而提高了测量方法的稳定性和灵敏度;对反应温度、停留时间、磷酸和溴酸钾的浓度和比例、泵的转速以及样品水蒸气蒸馏时间等试验条件及参数进行优化。结果:催化反应体系可在90℃以上进行,甲醛的测量线性范围为0.05～2μg/mL,相关系数(γ)大于0.999,方法的检出限为2.49ng/kg,加标回收率为96.5%～106%,相对标准偏差小于5%。结论:此法可用于测定水产品中的微量甲醛,该方法准确、可靠。Objective： A catalytic spectrophotometric method based on the oxidation of crystal violet by potassium bromate in the phosphoric acid medium was established for the determination of trace amounts of formaldehyde in aquatic products. Method： A de-bubbling device by home-made three-way joint （T-piece）was used in order to improve the ana- lytical stability and sensitivity. The amounts of formaldehyde in several kinds of seafood were measured by the established method at a higher reaction temperature （〉90 ℃）. The experimental conditions including the temperature of reaction, the stop time, the concentrations and ratios of phosphoric acid and potassium bromate, the flow rates and the distillation time of samples were optimized. Result： Under the optimum conditions, linear response of formaldehyde ranged from 0.05 to 2 mg/L（γ〉0.999）. The detection limit measured with ll replicates of procedure blank and estimated with 3-fold stan-dard deviation was 2.49 ng/kg. The recovery tests by adding standards ranged from 96.5% to 106%, with the relative standard deviation （RSD） less than 5%. Conclusion： With the merits of high sensitivity and precision, the method was suitable for the measurement of trace amounts of formaldehyde in aquatic products.

关 键 词：流动注射 催化光度法 甲醛 水产品 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]