海参体壁酸溶性胶原提取及氨基酸组成分析  

Study on the Extraction and Amino Acid Composition Analysis of Acid Soluble Collagen from the Body Wall of Sea Cucumber Stichopus japonicus

作　　者：高杨[1] 董秀萍[1,2] 肖桂华[1] 江慧敏[1] 朱蓓薇[1,2] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品与发酵工业》2008年第11期171-174,共4页Food and Fermentation Industries

基　　金：国家自然科学基金项目(30571449);科技部国际重大合作项目(2006DFA32580);国家重点基础研究发展计划(973)项目(2006CB708210)

摘　　要：以海参体壁为原料,采用乙酸溶剂法提取酸溶性胶原。以酸溶性胶原得率为指标,通过均匀设计试验确定了乙酸溶剂法提取酸溶性胶原的优化条件:温度为4℃,乙酸浓度为0.5 mol/L,料液比为1∶1 000,提取时间为72 h,在此条件下酸溶性胶原的得率可达到胶原纤维原料的7.35%(质量分数)。氨基酸组成分析表明,海参体壁酸溶性胶原的甘氨酸(Gly)含量为33.6%,羟脯氨酸(Hyp)含量为7.04%。The acid soluble collagen（ASC） was extracted by acetic acid from the body wall of the sea cucumber Stichopus japonicus. Taking yield rate of ASC as target, the optimum conditions were determined by the uniform design. It was found that the optimum conditions for the extraction of ASC were： temperature 4℃ ; acetic acid concentration 0.5 mol/L; dosage liquor ratio 1 ： 1000; extraction time 72 h. Under the optimum condition, the ASC yield rate from collagen fibrils was 7.35%. Amino acid composition analysis of ASC indicated that the content of Gly was 33.6% and Hyp was 7.04%.

关 键 词：海参 酸溶性胶原 提取 均匀试验 氨基酸组成 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]