杜仲籽油中α-亚麻酸的甲酯化方法优化  

Optimization of methyl esterification method of α-linolenic acid in Eucommia seed oil

作　　者：彭密军[1,2] 彭胜[1] 伍钢[1] 印大中[2] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000 [2]湖南师范大学生命科学学院,长沙410081

出　　处：《中国油脂》2009年第1期76-79,共4页China Oils and Fats

基　　金：湖南省科技厅项目“亚麻酸自由基氧化及其动力学研究”(编号:06FJ3010)

摘　　要：以超临界CO2萃取的杜仲籽油为原料,对其主要成分α-亚麻酸的甲酯化方法进行筛选和优化。在确定合适的α-亚麻酸甲酯化方法基础上,考察了催化剂用量、催化剂浓度、反应温度、反应时间等对α-亚麻酸甲酯化效果的影响,确定最佳的甲酯化条件为:用2 mL石油醚-苯(V(石油醚)∶V(苯)=1∶1)为溶剂将0.2 g杜仲籽油溶解,加入5 mL0.2 mol/L的KOH-甲醇溶液作催化剂,反应温度50℃,反应时间30 min。薄层展开检识显示在该条件下,杜仲籽油中α-亚麻酸甲酯化反应完全,气相色谱检测表明甲酯化率可达92.59%。The methyl esterification methods of Eucommia seed oil extracted by supercritical carbon dioxide were investigated, and the optimal methyl esterification version rate of α - linolenic acid in the oil. On the basis of the effects on α - linolenic acid methyl esterification of the method was selected by determining the conthe selection of methyl esterification method, dosage and concentration of catalyst, reaction time and temperature were discussed. The results showed that ： 0.2 g Eucommia seed oil was dissolved in 2 mL organic solvent mixed by petroleum ether and benzene at the volume ratio of 1 ： 1, subsequently 5 mL 0.2 mol/L KOH - methanol was added, then the reaction system was esterified at 50 ℃ for 30 min, and the conversion rate of α- linolenic acid in Eucommia seed oil reached 92.59%.

关 键 词：杜仲籽油 Α-亚麻酸 甲酯化 气相色谱法 薄层检识 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]