黑米皮花色苷的大孔树脂吸附纯化研究  

RESEARCH ON ADSORPTION CHARACTER AND PURIFICATION OF BLACK RICE ANTHOCYANINS WITH MACROPOROUS RESIN

作　　者：侯方丽[1,2] 张名位[2] 苏东晓[1,2] 张瑞芬[2] 魏振承[2] 张雁[2] 池建伟[2] 唐小俊[2] 

机构地区：[1]华中农业大学食品科学技术学院,武汉430070 [2]广东省农业科学院农业生物技术研究所农业部功能食品重点开放实验室,广东广州510610

出　　处：《华南师范大学学报（自然科学版）》2009年第1期100-104,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金(No:30671268);国家科技支撑计划(2006BAD27B09-2)

摘　　要：比较了5种大孔吸附树脂对黑米皮花色苷(Black Rice Anthocyanins,BRA)的吸附纯化效果,研究了AB-8型大孔树脂对BRA的吸附与解吸特性.结果表明:AB-8大孔树脂对BRA具有较好的吸附和解吸能力,是吸附纯化BRA的最佳树脂类型,其分离纯化BRA的最佳工艺参数为:上柱液pH值=2,样品质量浓度1.0 mg/mL,吸附流速1.0 mL/min,以体积分数为70%乙醇为解吸剂,洗脱速度为1.0 mL/min.树脂的重复利用率好,使用5次后吸附率无显著性差异(P>0.05),使用7次后,吸附率仅降低2.58%.BRA经AB-8大孔树脂纯化后,花色苷含量提高2.38倍,总抗氧化能力提高3.99倍.The absorption and pui：ification effects of 5 kinds of resins on Black Rice Anthocyanins （BRA） were compared, and the properties of adsorption and desorption for AB - 8 on BRA were further investigated. The result shows that AB -8 is the best resin for BRA due to its better adsorption and desorption capability. The optimal technical parameters of AB - 8 are as follows ： the pH of solution is 2.0 ; the anthoeyanins concentration of the sample solution is 1.0 mg/mL, and the adsorption flow rate is lmL/min; the desorption flow rate is 1. OmL/min with 70% ethanol as the desorptioner. The resin has a good reutilization. The desorption capability has no significant changes after using 5 times （ P 〉 O. 05 ）, and with only 2.58% reduction after using 7 times. After purified with AB - 8, the content of BRA increases 2.38 times and total antioxidant capability enhances 3.99 times.

关 键 词：黑米皮花色苷 AB-8大孔吸附树脂 吸附性能 

分 类 号：Q5[生物学—生物化学]