高耐性高产酒精酵母的分离和筛选  

Isolation and Screening of High-alchol-yield Yeast with Strong Tolerance

作　　者：张怀东[1,2] 刘作易[1,2,3] 周礼红[1] 杨坤[1] 朱国胜[2] 刘永翔[2] 万强[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省农业生物技术重点实验室,贵州贵阳550006 [3]贵州省农业科学院,贵州贵阳550006

出　　处：《酿酒科技》2009年第3期40-42,共3页Liquor-Making Science & Technology

基　　金：贵州省科技厅农业类攻关项目:红薯酒精生产技术研究(黔科合NY[2007]3024号)

摘　　要：采用含高浓度酒精培养基从自然界中分离到63株酵母,经过四级筛选,得到高耐性、高产酒精的酵母菌株Cor-01。该菌株耐酒精度20%vol,耐糖度22°Bix,致死温度为55℃。以红薯淀粉为原料,在料水比1∶2、30℃、100r/min、发酵72h的条件下,生料发酵,成熟醪酒精的体积分数达到16.2%。63 yeast strains were isolated from the nature by using the culture medium which contained high concentration of alcohol. After four-grade screening, one yeast strain, Cor-01, which had strong tolerance and could produce high-yield alcohol, was finally obtained. The alcohol tolerance of such strian was 20 %（v/v）, the sugar tolerance was 22°Bix, and the lethal temperature of such strain was at 55 ℃. Sweet potato starch was used as raw materials for uncooked material fermentation, the ratio of material to water was 1∶2, after 72 h fermentation at 30 ℃, 100 r/min, the ethanol concentration could reach up to 16.2 % in fermented mash.

关 键 词：酒精 酵母 分离 筛选 

分 类 号：TS261.1[轻工技术与工程—发酵工程]