乌骨鸡黑色素的高效液相色谱法测定  

Determination of Melanin from Black-bone Silky Fowl(Gallus gallus domesticus Brisson) by HPLC

作　　者：孙亚真[1] 谢明勇[1] 涂勇刚[1] 田颖刚[1] 黄林华[1] 李昌[1] 王远兴[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《分析测试学报》2008年第12期1363-1366,共4页Journal of Instrumental Analysis

基　　金：教育部长江学者和"创新团队发展计划"资助项目(IRT0540);江西省农业科技攻关重点项目(2005);江西省研究生创新基金资助项目(2007);南昌大学测试基金资助项目(2006)

摘　　要：建立了乌骨鸡黑色素氧化产物2,3-二羧酸吡咯(PDCA)和2,3,5-三羧酸吡咯(PTCA)的HPLC测定方法,对乌骨鸡中黑色素进行定量研究。采用Kromasil C18柱(250 mm×4.6 mm,5μm)分离,二极管阵列检测器检测,流动相为甲醇-0.1%甲酸,梯度洗脱,检测波长分别为282.8 nm和269.8 nm,并采用LC-MS对色谱峰进行定性。结果表明:PDCA和PTCA能得到很好的分离,经过初步分离纯化的乌骨鸡黑色素粗品中黑色素的含量为76.3279μg/mg。A HPLC method was established for the quantitative analysis of melanin from black-bone silky fowl（ Gallus gallus domesticus Brisson, BSF） by determination of pyrrole-2, 3-dicarboxylic acid （PDCA） and pyrrole-2, 3, 5-tricarboxylic acid（PTCA） which were oxidative products of melanin. The compounds were seperated in a Kromasil C18 column （4. 6 mm ×250 mm, 5 μm） using grandient elution with a mobile phase of 0. 1% formic acid -methanoll The analytes were monitored by the diode array detector at 282. 8 nm, respectively. Qualitative analysis was performed by LC -MS. The results showed that PDCA and PTCA had good resolution on HPLC and the content of melanin was 76. 327 9 μg/mg in crude melanin sample after separation and purification. The good selectivity, high recovery and reproducibility of the method made it be suitable for the determination of BSF melanin.

关 键 词：乌骨鸡黑色素 高效液相色谱 2 3 5-三羧酸吡咯 2 3-二羧酸吡咯 

分 类 号：O657.72[理学—分析化学] TS264.4[理学—化学]