用玉米粉生产纤维素酶的发酵工艺探讨  

Studies on Cellulase Production by Corn Flour

作　　者：林开江[1] 阮丽娟[1] 王龙英[1] 舒小洪[1] 

机构地区：[1]浙江省农业科学院,杭州310021

出　　处：《生物技术》1998年第2期31-34,共4页Biotechnology

摘　　要：本文报导了玉米粉为发酵原料，不用其他天然纤维素或改性纤维素生产纤维素酶的研究。30％的玉米粉以0．32－0．4％的HCl在125℃～128℃水解缩合反应2h后，稀释5倍作为纤维素酶发酵炭源，在三角烧瓶中进行二级发酵，取得滤纸酶活每毫升7个以上国际单位，配合其他因素，最高可达20个以上国际单位。The fermentation process of cellulase by corn and soybean cake flour without adding any naturai cellulose and its derivatives is reported in this paper. 30% corn flour in the solutionor 0. 32- 0. 4 % HCL was teated at 125℃ - 128℃ for 2 hours in flask. The syrup consisted of giucose and its condensates was diluted 5 times before being conducted two -stage fermentation in flasiks. A cellulase special activity of 7 IU /ml of FPA was obtatined. A 20 IU / ml of FPA has been detected in fermentation broth with other factors that are good for cellulase production.

关 键 词：纤维素酶 玉米粉 发酵工艺 

分 类 号：TQ925[轻工技术与工程—发酵工程]