泰和乌骨鸡黑色素的体外抗氧化作用  

The Antioxidant Activity of Melanin from Taihe Black-Bone Silky Fowl in vitro

作　　者：涂勇刚[1] 孙亚真[1] 田颖刚[1] 杨美艳[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品与生物技术学报》2009年第2期145-149,共5页Journal of Food Science and Biotechnology

基　　金：教育部“长江学者和创新团队发展计划资助”项目(IRT0540);江西省农业科技攻关重点项目(2005);江西省研究生创新基金项目(YC07A034)

摘　　要：采用分光光度法测定磷钼酸配合物、自由基和丙二醛(MDA)的含量,用于研究酶法提取的泰和乌骨鸡黑色素的总抗氧化能力及其清除自由基和抗脂质过氧化的作用,研究过程中用合成黑色素与之进行比较。结果表明:泰和乌骨鸡黑色素清除羟基自由基的能力虽然低于合成黑色素,但其总抗氧化能力与合成黑色素相当,而清除DPPH自由基和超氧阴离子自由基的能力以及抗脂质过氧化作用都优于合成黑色素,说明泰和乌骨鸡黑色素具有明显的体外抗氧化功能。The antioxidant activity of melanin from Taihe Black-bone Silky Fowl （TBSF） in vitro was studied. Total antioxidant activity, radical scavenging activity and inhibition of lipid peroxidation were evaluated by the content of phosphomolybdenum complex, radical and malondialdehyde （MDA） observed in the spectrophotometric method. Those various antioxidant activities were compared to the synthetical melanin. It was found that the ability of TBSF melanin on scavenging hydroxide free radicals was weaker than that of the synthetical melanin and total antioxidant activity were quite close for both pigments, but superoxide radical scavenging activity, DPPH radical scavenging activity and prevention of lipid peroxidation of TBSF melanin were all superior than that of the synthetic melanin. The results demonstrated that TBSF melanin has stronger antioxidant effects in vitro.

关 键 词：泰和乌骨鸡 黑色素 清除自由基 抗脂质过氧化 抗氧化作用 

分 类 号：S831[农业科学—畜牧学]