黄曲霉毒素B1检测ELISA条件的优化  

Optimization of ELISA Method Conditions for Detection of AFB1

作　　者：才琳[1] 张甄[1] 钱丽丽[1] 张园园[1] 裴世春[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《农产品加工（下）》2009年第3期69-72,共4页Farm Products Processing

基　　金：黑龙江省教育厅项目(1151hz025);中国博士后科学基金资助项目(20070410882)

摘　　要：采用黄曲霉毒素B1与牛血清白蛋白偶联物(AFB1—BSA)抗原免疫Balb/C小鼠获得特异性抗体。以AFB1—STI为检测抗原,利用此抗体,进行间接竞争ELISA法试验。通过正交实验确定了最佳抗原包被反应质量浓度为1.0μg/mL;最佳抗原包被条件为:4℃过夜,一抗和酶标二抗IgG—HRP;最佳稀释度为1∶200000和1∶5000。在此优化条件下制备的抗体检测黄曲霉毒素B1具有很高的特异性和灵敏性,具有实际应用价值。The specific antibody was obtained by immune Balb/C mice with Aflatoxin Bl-bovine serum albumin （BSA） conjugate （AFB1-BSA） . The AFB1-STI was used to detected antigen, using this antibody, a indirect competitive ELISA method was used the orthogonal experiment of four factor and three level for the detection of AFB1 was developed with the best concentration of antigen, antigen coating condition, monoclonal antibody and second antibody marked with HRP being of 1.0 μg·mL-1, 4 ℃ overnight 1 ： 200 000, and 1 ： 5 000, respectively. The antibody prepared by the optimal conditions, was highly specific and sensitive on detecting AFB1, showing high practical application value.

关 键 词：黄曲霉毒素B1 酶联免疫吸附测定 单克隆抗体 

分 类 号：R446.61[医药卫生—诊断学]