几株脂肪酶产生菌的筛选及生长特性的研究  

Screening of lipase-producing bacteria and growth behaviour

作　　者：熊杰[1,2] 王仿[1,2] 程模香[1,2] 孙燕飞[1,2] 邓磊[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]新疆兵团绿洲生态农业重点实验室,新疆石河子832003

出　　处：《中国酿造》2009年第4期65-67,共3页China Brewing

基　　金：石河子大学科学技术研究发展计划(RCZX200527)

摘　　要：从食用油加工厂含油土壤等样品中分离筛选产脂肪酶菌株,得到活性较高的D7、D14、D20株菌,对其生长和产酶条件进行了初步研究。结果表明,3菌株均在培养24h左右出现生长高峰,D7和D20株菌在培养48h后表现出高的酶活性,而D14株菌则在12h^27h活性较高,3菌株最适pH值均为6~7,最适生长温度均为28℃~30℃。Strains D7, D14 and D20 with high lipase production were selected from lipase-producing bacteria isolated from soil sample nearby oil refinery. Growth and enzyme production affected by fermentation conditions was studied. It is showed that the highest growth rate of three strains occurred after about 24 h fermentation. For strains D7 and D20, enzymatic activity reached the highest when fermented for 48h, and for D14 it lasted in the duration of 12-27h. For three strains, the optimum pH and temperature were 6-7 and 28-30℃, respectively.

关 键 词：微生物 脂肪酶 筛选 发酵 

分 类 号：Q93-331[生物学—微生物学]