一株深海热液口超嗜热古菌的分类鉴定及高温酶活性研究  

Identification and hyperthermophilic enzyme activities of a hyperthermophilic archaeon isolated from deep-sea hydrothermal vent

作　　者：王淑军[1,2] 陆兆新[1] 吕明生[2] 刘红飞[2] 徐金利[2] 杨磊[2] 

机构地区：[1]南京农业大学食品科学技术学院,江苏南京210095 [2]淮海工学院江苏省海洋生物技术重点建设实验室／食品工程学院,江苏连云港222005

出　　处：《南京农业大学学报》2009年第2期130-136,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(40746030);江苏省“六大人才高峰”第三批资助项目(06-A-017);江苏省教育厅自然科学基金项目(06KJB550004);江苏省海洋生物技术重点建设实验室项目(2006HS008);连云港市自然科学基金项目(KK06076)

摘　　要：对一株分离自深海热液口古菌HJ21进行了分类鉴定及高温酶活性的研究。该菌株是极端嗜热的厌氧球菌，直径为1．0～1．2μm。菌株最适生长温度88℃；菌株生长pH为5．0—9．0，最适pH为6．5～7．0；菌株生长NaCI质量浓度为10～50g·L^-1，最适为20g·L^-1。根据其形态特征、生理生化特性以及16S rRNA基因序列分析结果，确定HJ21菌株为热球菌属（Thermococcus）。该菌株能产生高热稳定的高温α-淀粉酶、普鲁兰酶、α-葡萄糖苷酶和蛋白酶，这些酶的最适作用温度分别为95、95、100和100℃，α-淀粉酶、普鲁兰酶和α-葡萄糖苷酶在90℃的半衰期分别为5、5和2h；蛋白酶在100℃保温2．5h后仍具有84％的酶活性。The identification and hyperthermophilic enzyme activities of an archaeon from a deep-sea hydrothermal vent were studied. The extremely thermophilic anaerobic archaeon strain H J21 was irregular cocci with 1.0 - 1.2 μm in diameter. The optimum temperature of growth was 88 ℃. The pH range for growth was 5.0 -9. 0, with an optimum pH of 6. 5 -7.0. The NaCI concentration range for growth was 10 -50 g · L^-1, with an optimum concentration of 20 g · L^-1. It belonged to the genus Thermococcus based on the 16S rRNA gene sequence and morphological, biochemical and physiological characteristics. The strain HJ21 produced thermostable eL-amylase, pullulanase, α-glucosidase and protease. The optimal working temperatures of the α-amylase, pallulanase, α-glucosidase and protease were 95, 95, 100 and 100℃, respectively. The half-life of the a-amylase, pullulanase, α- glueosidase were 5, 5 and 2 h at 90 ℃ , respectively; 84% of the protease remained active after incubation at 100℃ for 2. 5 h.

关 键 词：深海热液口 超嗜热古菌 16S RRNA Α-淀粉酶 普鲁兰酶 Α-葡萄糖苷酶 蛋白酶 

分 类 号：Q939.13[生物学—微生物学]