一株降胆固醇作用乳酸菌KF5的分离与鉴定  

Screening and Identification of Cholesterol-Reducing KF5 Strain

作　　者：张玢[1] 许女[1] 习傲登[1] 王艳萍[1] 

机构地区：[1]天津市食品营养与安全重点实验室天津科技大学食品工程与生物技术学院,天津300457

出　　处：《天津科技大学学报》2009年第2期17-20,共4页Journal of Tianjin University of Science & Technology

基　　金：国家"十一五"科技支撑计划项目(2006BAD04A06)

摘　　要：利用邻苯二甲醛法,从源于西藏Kefir粒的56株乳酸菌和实验室保存的9株乳酸菌中,筛选得到了一株降胆固醇效果明显的乳酸菌KF5.经生理生化实验及分子遗传学鉴定,该菌株为植物乳杆菌(Lactobacillus plantarum).为了能够进一步研究KF5的降胆固醇功能,对其进行了生物学特性的考察.结果表明,KF5具有较好的耐酸性以及一定的胆盐耐受力.The cholesterol-reducing ability was tested on 56 strains of lactic acid bacteria isolated from Tibet kefir grain and 9 strains of lactic acid bacteria stored in our lab by the o-phthalaldehyde method. KF5 strain was screened for its better cho- lesterol-reducing ability and bionomics. KF5 strain was identified as Lactobacillus plantarum based on morphologic observe,biochemical identification and sequence analysis of 16S rDNA. The further study on the bionomics of KF5 strain was carried out. The results show that KF5 strain has good acid tolerance and bile salt tolerance.

关 键 词：植物乳杆菌 胆固醇 筛选 鉴定 生物学特性 

分 类 号：Q93-3[生物学—微生物学]