短小芽孢杆菌2080产碱性蛋白酶的扩大培养  

Scale up of production of alkaline protease from Bacillus pumilus 2080

作　　者：李祖明[1] 李鸿玉[1] 王德良[2] 王童[1] 白志辉[3] 李丽云[1] 

机构地区：[1]北京联合大学师范学院,北京100011 [2]中国食品发酵工业研究院,北京100027 [3]中国科学院生态环境研究中心,北京100085

出　　处：《食品科技》2009年第4期14-17,共4页Food Science and Technology

基　　金：北京市教委科研计划项目(KM200811417006);北京市属市管高校人才强教计划资助项目;北京市优秀人才培养资助项目(20061D0502200295)

摘　　要：采用5L发酵罐研究短小芽孢杆菌(Bacillus pumilus)2080产碱性蛋白酶的扩大培养。结果表明,在较优的扩大培养条件下,即空气流量2.5L/min、空气压力0.08MPa和搅拌速度500r/min,短小芽孢杆菌2080碱性蛋白酶的产率较摇瓶水平提高了28%,达到5.17×106U/mL,而发酵时间缩短为42h。This paper describes scale up of production of alkaline protease from Bacillus pumilus 2080 in a 5 L ferrnentor. The maximum alkaline protease yield of 5.17×10^6 U/mL from Bacillus pumilus 2080 was obtained after scale up of production of alkaline protease in a 5 L fermentor with the airflow rate of 2.5 L/min, the air pressure of 0.08 MPa, and the agitation rate of 500 r/min, which was increased by 28% in contrast with that in shake flasks. Meanwhile the fermentation time was reduced to 42 h, which was half of that in shake flasks.

关 键 词：短小芽孢杆菌 碱性蛋白酶 扩大培养 

分 类 号：TS201.3[轻工技术与工程—食品科学]