里氏木霉β-葡萄糖苷酶水解大豆异黄酮糖苷的工艺研究  

Hydrolysis of Soybean Isoflavone Glucosides by β-Glucosidases from Trichoderma Reesei

作　　者：许晶[1] 孙艳梅[1] 张永忠[1] 

机构地区：[1]东北农业大学理学院应用化学系,哈尔滨150030

出　　处：《中国粮油学报》2009年第3期31-34,共4页Journal of the Chinese Cereals and Oils Association

基　　金：黑龙江省"十一五"重大科技攻关项目(GA06B402-4);大豆生物学教育部重点实验室主任基金项目(SB05A06)

摘　　要：研究了里氏木霉β-葡萄糖苷酶的酶学性质，及其水解大豆异黄酮糖苷能力。该酶反应的最适温度为50℃，最佳pH为5．0；此酶在pH4．5～5．5之间和低于60℃下酶较稳定，在80℃基本无活力。采用里氏木霉β-葡萄糖苷酶水解的方法，将大豆异黄酮糖苷转化为苷元，以染料木苷水解率为检测指标。通过正交试验确定了该酶水解大豆异黄酮的工艺奈件为反应温度50℃、水解时间90min、水解介质pH4．5、加酶量20U，大豆异黄酮中染料木苷的水解率为99％。Characterization of β- glucosidase from Trichoderma Reesei was studied. As showing in results, the enzyme exhibits optimal activity with p - nitrophenyl- β-D-glucoside at 50℃ and pH 5.0, stable activity in pH 4.5-5. 5 and temperature 〈 60℃, but is basically inactivated at 80℃. Soybean isoflavone glucoside was hydrolyzed into aglycon by β-glucosidase from Trichederma Reesei. Using genistein content as assessable index, the enzyanatic hydrolysis condition optimized through orthogonal test is as follows： temperature 50℃, time 90 rain, pH 4.5, enzyme dose 20 U. Under this optimal condition, the hydrolysis rate of genistein in soybean isoflavone glucoside reaches almost 99%.

关 键 词：里氏木霉β-葡萄糖苷酶 大豆异黄酮糖苷 酶水解 

分 类 号：TS201.1[轻工技术与工程—食品科学]