酿酒酵母在高渗条件下分泌蛋白的研究  

Study on Protein Secretion from Saccharomyces Cerevisiae Under High Osmotic Pressure

作　　者：姜宗豹[1,2] 苗君[1] 赵睿[1] 栾静[1] 赵长新[1] 

机构地区：[1]辽宁省发酵工程重点实验室,大连工业大学生物与食品工程学院,辽宁大连116034 [2]华润雪花啤酒(朝阳)有限公司,辽宁朝阳122000

出　　处：《酿酒科技》2009年第5期22-25,28,共5页Liquor-Making Science & Technology

基　　金："十一五"国家科技支撑计划;项目编号:2007BAK36B01

摘　　要：以酿酒酵母2144为研究对象,利用不同葡萄糖浓度的YNB(全氨基酸)培养基,通过Sephadex G-25和Sephadex G-75分离酵母胞外分泌蛋白,最后采用SDS-PAGE进行分析。结果表明,高糖浓度下16h分泌蛋白出现了高分子量蛋白,经SDS-PAGE测定分子量为53KDa与酵母蛋白酶A酶原分子量一致,分泌的低分子量蛋白与正常对照组差异性很大。Saccraomyces cerevsiae 2144 was cultured in YNB culture medium of different glucose concentration. The secreted protein from Saccharomyces cerevisiae was separated by Sephadex G-25 and Sephadex G-75 ,and then analyzed by SDS-PAGE. The results showed that high molecular weight protein was secreted at 16 h under high concentration of glucose, and SDS-PAGE indicated that the protein molecular weight was 53KDa, the same as yeast protein A zymogen. However, low molecular weight proteins were quite different from normal series in contrast groups.

关 键 词：酿酒酵母 高渗条件 分泌蛋白 

分 类 号：TS261.11[轻工技术与工程—发酵工程]