酿酒酵母产乙醛脱氢酶发酵条件的研究  

Study on the Fermentation Conditions for Aldehyde Dehydrogenase Production by Saccharomyces cerevisiae

作　　者：吴桂英[1] 金放[2] 吴元欣[2] 

机构地区：[1]黄石理工学院化学与材料工程学院,湖北黄石435003 [2]武汉工程大学化工与制药学院,湖北武汉430073

出　　处：《酿酒科技》2009年第5期26-28,共3页Liquor-Making Science & Technology

基　　金：湖北省国家科技合作重点项目计划(006CA013);黄石理工学院院级项目(08yjz13Q)

摘　　要：选择了一株酿酒酵母(Saccharomyces cerevisiae)菌As2.399,通过单因素和正交实验得到酿酒酵母产乙醛脱氢酶(Aldehyde Dehydrogenase,ALDH)的最优发酵条件。结果表明,产ALDH的最优培养条件为温度30℃、pH值为6、通气量为200mL、接种量为7%。液体发酵培养中pH值对ALDH活性的影响最大。A strain yeast Saccharomyces cerevisiaen As2.399 was selected to produce ALDH. The fermentation conditions of yeast were optimized by single factor and orthogonal test. The results showed that high quality ALDH could be produced when the temperature was at 30 ℃,the initial pH value was 6.0, and 20 mL air flow and 7 % inoculation quantity.

关 键 词：酿酒酵母 乙醛脱氢酶 发酵 

分 类 号：TS261.11[轻工技术与工程—发酵工程]