硫酸酯化猴头菌多糖的结构与构象分析  

Structure and Conformation Analysis of Sulfated Polysaccharide from the Fruiting Bodies of Hericium erinaceus

作　　者：张安强[1] 马新[1] 何荣军[1] 葛青[1] 杨开[1] 孙培龙[1] 张劲松[2] 

机构地区：[1]浙江工业大学生物与环境工程学院 [2]上海市农业科学院食用菌研究所,上海201106

出　　处：《食品与发酵工业》2009年第3期64-67,共4页Food and Fermentation Industries

摘　　要：利用碱提醇沉法从猴头菌(Hericium erinaceus)子实体中获得水不溶性多糖HEPFA,氯磺酸-吡啶法对HEPFA进行硫酸化修饰,制得能溶于水的硫酸化猴头菌多糖(S-HEPFA);通过DEAE-Sepharose Fast Flow离子交换柱层析和Sephacryl S-500凝胶柱层析对S-HEPFA进行纯化;运用化学方法和波谱技术对S-HEPFA进行结构分析。碱提醇沉得到相对分子量为5.38×105u,硫酸基含量为19.5%的硫酸酯化的猴头菌多糖(S-HEPFA);红外光谱分析表明,在波数1236cm-1、820cm-1处有硫酸酯键的特征吸收峰;离子色谱检测,该组分只含有葡萄糖,甲基化分析得S-HEPFA糖链主要的葡萄糖残基由1-连接Glcp、1,3-连接Glcp和1,3,6-连接Glcp组成,摩尔比为2.07:1:1.83;刚果红实验分析得S-HEPFA在水溶液中或微碱溶液中为三股螺旋构象。Aim structure and conformation analysis of sulfated polysaccharide from the fruiting bodies of Hericium erinaceus. Methods Water-insoluble polysaccharide HEPFA was extracted with 1 mol/L NaOH from the fruiting bodies of Hericium erineceus. A water-soluble sulfated derivative S-HEPFA was obtained from HEPFA with chlorosulfonic acid-Pyridine, and purified by DEAE-Sepharose Fast Flow ion exchange chromatography and Sephacryl S-500 High Resolution column chromatography. The structure of the S-HEP- FA was then determined by a combination of chemical methods and use of IR spectroscopy. Results and conclusion sulfate content of the S-HEPFA was 19.5% and its average molecular weight was 5.38 × 10^5 u. The IR spectrum of S-HEPFA showed the characteristic absorptions of sulfate ester bond at 1 236 cm ^-1 and 820 cm^-1. The S-HEPFA consisted of only glucose by ion chromatogram. Methylation analysis of the S-HEPFA revealed 1-1inked glucose, 1,3-1inked glucose and 1,3,6-1inked glucose in the ratios 2.07 ： 1 ： 1.83. The Congo red test showed that S-HEPFA in neutral or weak base solution takes a triple-helical conformation.

关 键 词：猴头菌 硫酸化多糖 结构与构象分析 

分 类 号：R284.1[医药卫生—中药学]