拉曼光谱法快速检测掺入梨汁的浓缩苹果汁  

Rapid Determination of Apple Juice Mingled with Pear Juice by Raman Spectroscopy

作　　者：马寒露[1,2] 董英[1] 张孝芳[2] 张新智 齐小花[2] 邹明强[2] 张卓勇[4] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]中国检验检疫科学研究院,北京100123 [3]陕西出入境检验检疫局,陕西西安710068 [4]首都师范大学化学系,北京100037

出　　处：《分析测试学报》2009年第5期535-538,共4页Journal of Instrumental Analysis

基　　金：质检公益性行业科研专项资助项目(10-40,2007GYJ033);国际科技合作与交流专项资助项目(2008DFA40270);国家质检总局科研项目

摘　　要：应用便携式拉曼光谱仪结合化学计量学技术建立了浓缩苹果汁掺入梨汁的快速检测方法。实验表明,苹果汁和梨汁的拉曼特征频率相同,给出了特征频率及其对应的分子振动模式。发现苹果汁和梨汁在866cm-1和1126cm-1处的拉曼光谱有微小差别,这可能由于苹果汁和梨汁中果糖异构体含量不同所致。用支持向量分类机(SVC)中的4个核函数对19个苹果汁、梨汁样本进行有效鉴别,准确率达100%,并使用支持向量回归机(SVR)对掺入不同含量梨汁的苹果汁样本进行建模预测,大部分样品相对误差在10%以内。A method for the rapid determination of apple juice mingled with pear juice was proposed by a portable Raman spectrometer combined with chemometrics. The Raman shift for pear juice was the same as that for apple juice, and the functional groups and vibrational modes obtained for the Ra- man spectra of fruit juice were given. Slight differences of the Raman signals at 866 cm -1 and i 126 -1 cm were found between the spectra of apple juice and pear juice, which may be due to the differ- ence of fructose isomers content. Based on the presented method, 19 apple juices and pear juices were easily distinguished by four different kernel functions of support vector machines （SVM）. A mathematical model with SVM was established to analyze the content of pear juice added to apple juice. The relative errors for most of the analyzed samples were less than 10% .

关 键 词：拉曼光谱法 浓缩苹果汁 梨汁 支持向量机 快速检测 

分 类 号：O657.37[理学—分析化学] TS255.44[理学—化学]