带鱼(Trichiurus lepturus)下脚料蛋白水解物的成分分析及抗高血脂功效的研究  

NUTRITIONAL INGREDIENT AND ANTIHYPERLIPIDEMIA ACTION OF PROTEIN HYDROLYSATES FROM HAIRTAIL TRICHIURUS LEPTURUS WASTE

作　　者：谢超[1] 邓尚贵[1] 夏松养[1] 霍健聪[1] 

机构地区：[1]浙江海洋学院食品与药学学院,舟山316000

出　　处：《海洋与湖沼》2009年第3期307-312,共6页Oceanologia Et Limnologia Sinica

基　　金：浙江省重大科技专项资助;2007C12013号

摘　　要：采用化学分析、氨基酸组成分析及动物体内实验等方法,研究了带鱼下脚料蛋白水解物的一般成分、氨基酸组成和抗高血脂作用,以期为带鱼及其下脚料蛋白水解物的高效利用提供资料。结果表明,带鱼下脚料蛋白水解物含蛋白质17.16%、脂肪9.08%、水分71.61%、灰分1.12%。在必需氨基酸中,异亮氨酸、亮氨酸、赖氨酸、蛋氨酸和胱氨酸、苏氨酸、色氨酸及缬氨酸达到FAO/WHO(1973)提出的理想氨基酸模式中相应氨基酸的88%—100%。动物体内实验结果表明,该蛋白水解物能显著抑制由高脂类饲料喂养导致的高血脂大鼠的TC、TG、HDL升高。带鱼下脚料蛋白水解物具有抑制高血脂动物血脂的升高,其抗高血脂作用可能源于它本身的功能因子,包括牛磺酸、DHA、EPA、Ca、P和Fe等。To evaluate the potential industrial application of hairtail-waste-yielded hydrolysate, amino acid composition and in vivo anti-hyperlipidemia action of the hydrolysate were investigated. Chemical analysis results show that the protein hydrolysate contained 17.16% protein, 9.08% fat, 71.61% moisture and 1.12% ash. Compared with the amino acid profiles recommended by FAO/WHO, the quality of the protein hydrolysate is high, as it is rich in essential amino acids, including isoleucine, leucine, lysine, methionine and cystine, threonine, tryptophan, and valine, which covered 88%--100% of the FAO/WHO recommended. Animal test results reveal that the protein hydrolysate inhibited the rat hyperlipidemia caused by high lipid feedstuff in rats, including decrease in triglyceride, cholesterol, and dense lipoprotein. The hydrolysate protein of hairtail waste has anti-hyperlipidemia action, which might be due to its functional factors （including taurine, DHA, EPA, calcium, phyosphorus and iron）.

关 键 词：带鱼下脚料 蛋白水解物 氨基酸组成 抗高血脂 

分 类 号：Q53[生物学—生物化学]