Enzymatic treatment of estrogens and estrogen glucuronide  

作　　者：Takaaki Tanaka Toshiyuki Tamura Yuuichi Ishizaki Akito Kawasaki Tomokazu Kawase Masahiro Teraguchi Masayuki Taniguchi 

机构地区：[1]Department of Materials Science and Technology,Niigata University [2]Venture Business Laboratory,Niigata University [3]Department of Chemistry and Chemical Engineering,Niigata University

出　　处：《Journal of Environmental Sciences》2009年第6期731-735,共5页环境科学学报（英文版）

基　　金：supported by the grant from Sasaki Environment Technology Foundation to T.Tanaka

摘　　要：Natural and synthetic estrogens from sewage treatment systems are suspected to influence the reproductive health of the animals in the rivers. In this article, we investigated the enzymatic treatment of three estrogens （estrone, 17β-estradiol, and 17α-ethynyletstradiol） by a fungal laccase which oxidize phenolic compounds with dissolved oxygen. The elimination of the estrogenic activities by enzymatic oxidation was demonstrated by medaka vitellogenin assay. In addition, we developed an enzymatic treatment system comprised of β-D-glucuronidase and the laccase for 17β-estradiol 3-（β-D-glucuronide） degradation. The two enzymes eliminated 17β-estradiol 3-（β- D-glucuronide） and the intermediate, 17β-estradiol, efficiently.Natural and synthetic estrogens from sewage treatment systems are suspected to influence the reproductive health of the animals in the rivers. In this article, we investigated the enzymatic treatment of three estrogens （estrone, 17β-estradiol, and 17α-ethynyletstradiol） by a fungal laccase which oxidize phenolic compounds with dissolved oxygen. The elimination of the estrogenic activities by enzymatic oxidation was demonstrated by medaka vitellogenin assay. In addition, we developed an enzymatic treatment system comprised of β-D-glucuronidase and the laccase for 17β-estradiol 3-（β-D-glucuronide） degradation. The two enzymes eliminated 17β-estradiol 3-（β- D-glucuronide） and the intermediate, 17β-estradiol, efficiently.

关 键 词：enzymatic treatment laccase ESTRONE ESTRADIOL ETHYNYLESTRADIOL GLUCURONIDASE GLUCURONIDE 

分 类 号：X132[环境科学与工程—环境科学]