高效凝胶渗透色谱法检测菊糖相对分子质量  

HPGPC Determination of Relative Molecular Mass of Inulin

作　　者：张泽生[1] 张建平[1] 史珅[1] 史佳宁[1] 张婕[1] 

机构地区：[1]天津科技大学食品添加剂与营养研究室,天津300457

出　　处：《食品科学》2009年第12期219-221,共3页Food Science

摘　　要：为了建立利用高效凝胶渗透色谱测定菊糖相对分子质量的方法,采用GPC色谱柱,示差折光检测器检测,以三蒸水为流动相,流速0.8ml/min,柱温30℃。结果表明不同分子量标准右旋糖酐的保留时间与相应的相对分子质量对数值(lgMw)在相对分子质量1.0×103～2.5×104范围内具有良好的线性关系(r=0.9894),精密度实验RSD为0.24%,重现性实验RSD为0.21%,稳定性实验RSD为0.38%,准确度实验相对误差在0.4%左右。该法简便、快速、灵敏度高,精密度、重现性及稳定性均较好。用此法检测热回流、超声、微波三种提取工艺得到的菊糖相对分子质量分别为:热回流提取3500,超声提取2405,微波提取3864。A high performance gel permeation chromatography （HPGPC） method was developed for the determination of relative molecular mass of inulin. Separation was performed on a GPC column with triple distilled water as a mobile phase at the flow rate of 0.8 ml/min and 30℃ and determination was carried out using a refractive index detector. A direct linear relationship within the range of 1.0 ×10^3-2.5×10^5 of relative molecular mass （r = 0.9894） was found between retention time of dextranum and lgMw. Insulins obtained from jerusalem artichoke by hot water reflux method and ultrasonic-assisted and microwave-assisted Water extraction methods possessed varying relative molecular mass of 3500, 2405 and 3864, respectively. Methodology evaluation experiments revealed that the RSDs for precision, repeatability and stability were 0.24%, 0.21%, and 0.38%, respecitively, and the relative error for accuracy was around 0.4%. This method is proved to be fast, simple, sensible and repeatable.

关 键 词：菊糖 高效凝胶渗透色谱 相对分子质量 测定 

分 类 号：TS245.9[轻工技术与工程—制糖工程]