猪肚木叶中多酚含量测定及抗氧化活性研究  

Study on determination of total polyphenolic content and antioxidant activity of the leaves from Canthium horridum Bl.

作　　者：宋鑫明[1,2] 陈光英[1] 尹文清[2] 王安伟[1,2] 张永强[1] 谢东霞[1] 

机构地区：[1]海南省热带药用植物化学重点实验室,海南师范大学化学系,海南海口571158 [2]广西师范大学药用资源化学与药物分子工程教育部重点实验室,广西桂林541004

出　　处：《食品工业科技》2009年第6期91-93,共3页Science and Technology of Food Industry

基　　金：教育部科技重点项目(207090);海南省教育厅高等学校科研项目(Hjkj2008-28)

摘　　要：采用紫外分光光度法,以焦性没食子酸为标准品,在波长540nm处,对猪肚木叶提取物中总多酚类化合物含量进行了测定,并用流动注射化学发光法研究了猪肚木叶的抗氧化性能。实验结果表明,猪肚木叶中总多酚含量达0.4879%,标准曲线的回归方程Y=16.76X-0.0948,r=0.9997,RSD=0.745%;猪肚木叶具有较强的自由基清除能力,其IC50为0.35mg/mL。Method of UV-spectrophotometry was used at the wavelength of 540nm to quantify total polyphenol of the leaves from Canthium horridum BI. ,taking pyrogallic acid as standard.This thesis also determined antioxidation of the leaves of Canthium horridum BI.By flow injection chemiluminescence method using luminol-H2O2 system.The results showed as follows ： the content of polyphenols was 0.4879%.The regression equation with a good linearity （r =0.9997） between the absorbance and concentration was found. RSD was 0.745%. The leaves of Canthium horridum BLhad strong antioxidant activity.The IC50 values were found to be 0.35mg/mL.

关 键 词：猪肚木 流动注射化学发光 抗氧化性 多酚 紫外分光光度法 

分 类 号：TS201.2[轻工技术与工程—食品科学]