酱油工业生产菌沪酿3042基因工程转化体系的构建  

Construction of genic engineering transforming system of Aspergillus oryzae HuNiang 3042 used in soy sauce industry

作　　者：李方方[1] 潘力[1] 曾沛斌[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《食品工业科技》2009年第6期94-95,99,共3页Science and Technology of Food Industry

基　　金：广东省科技计划项目(2007A010900001)

摘　　要：以工业酿造常用菌株米曲霉沪酿3042为出发菌株,采用紫外诱变和氯酸钾压迫相结合的方法,得到硝酸盐营养缺陷型(niaD-)宿主菌,将含有niaD基因的载体转化到该宿主中,转化率为0.7个转化子/μgDNA,相对比较低,但转化子传代数次后遗传仍很稳定,该转化体系可用于工业生产菌沪酿3042的遗传改造。Aspergillus oryzae HuNiang 3042 was used as starting strain, and the host mutant strain with nitrate auxotrophic was screended by UV-induction and kalium chloricum resistance.The vector with the niaD gene coding for nitrate reductase was transfered to host strain.The transformants can use sodium nitrate as sole nitrogen source.The frequency of transformation was relatively low （0.7 transformants/μg DNA）, but the transformed phenotype of transformants was extremely stable for many generations without selective pressure. So this transformation system can be applied for genetics modification of Aspergillus oryzae HuNiang 3042.

关 键 词：米曲霉 niaD^- 筛选 转化 

分 类 号：TS264.21[轻工技术与工程—发酵工程]