酶解淡水鱼鳞制备水解胶原蛋白的研究  

Preparation of hydrolisis collagen from freshwater scales by enzymatic hydrolysis

作　　者：刘艳[1] 刘章武[1] 唐婧苗[1] 吴绍武[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023

出　　处：《武汉工业学院学报》2009年第2期11-14,17,共5页Journal of Wuhan Polytechnic University

摘　　要：测定了几种常见淡水鱼鳞的基本成份和氨基酸组成,研究了酶解鱼鳞制备水解胶原蛋白的工艺,并采取正交实验对水解条件进行了优化。结果表明淡水鱼鳞主要由蛋白质和灰分组成,粗蛋白含量高达55%以上;氨基酸组成显示淡水鱼鳞羟脯氨酸含量丰富,是制备水解胶原蛋白的良好原料。正交实验结果表明:55℃、酶与底物比(E/S)为3%、酶解2 h、底物浓度100 g/L时水解效果最好,此时,水解产物分子量较小,且分布集中。In this paper, the basic composition and amino acid ot common lreslawater scales were uetermlneu, and the enzymatic hydrolysis technology of collagen were optimized. The results indicated that freshwater scales were mainly composed of protein （more than 55% ） and inorganic substance. The composition of amino acid showed that freshwater scales were rich in Hydroxy Proline. The optimal conditions have been found by orthogonal tests as follows： concentration of the substrate 100g/L, hydrolyzing temperature 55℃, ratio of enzyme to substrate 3.0% ,hydrolyzing time 2 h. In this condition, the molecular weight of Hydrolisis collagen is small and the distribution is centralized.

关 键 词：淡水鱼鳞 酶解 胶原蛋白 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]