脉冲电场辅助提取花色苷及其影响  

Effect of PEF on Extraction of Anthocyanin

作　　者：罗炜[1] 张若兵[1] 王黎明[1] 陈杰[1] 廖小军[2] 莫孟斌[1] 关志成[1] 

机构地区：[1]清华大学深圳研究生院,深圳518055 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《高电压技术》2009年第6期1430-1433,共4页High Voltage Engineering

基　　金：国家“十一五”科技支撑计划(2006BAD05A02)~~

摘　　要：高压脉冲电场技术(pulsed electric field)是一种非热处理技术,它因能对生物细胞的细胞膜造成不可恢复的损伤而可应用于辅助提取细胞内物质。为此以红莓为实验材料,研究了PEF辅助提取法对红莓中花青素提取率的影响,并考察了在提取过程中PEF对花青素的降解作用。实验结果表明:PEF处理破坏了红莓果细胞的细胞膜,增大了细胞膜的通透性,从而增大了花色苷由细胞内向细胞外的传质过程,有效地提高了花色苷的提取率,并缩短了提取时间,提取率随着处理脉冲个数的增加而增大。在提取过程中,PEF对目标提取物Cy-3-gly具有显著的降解作用,且降解作用随着处理场强的增大及处理脉冲个数的增多而增大,PEF处理后的Cy-3-glu与对照样品具有相似的光谱特征,但在519nm处的吸收降低,333nm处的吸收稍有增大,这表明PEF处理使得Cy-3-glu生成了查尔酮类化合物。Pulsed electric field （PEF） is a novel non-thermal process technology. PEF treatment caused an irreversible disintegration on the cellular membrane of raspberry cell ,and the extraction yield of anthocyanins from raspberry was linearly correlated to the treatment pulses of PEF. During the extraction, Cyaniding-3-glucoside, the major anthocyanins in raspberry was found to be significantly degraded by PEF. The degradation effects of Cyaniding-3-glucoside decreased with the increase of the electric field or treatment time. In the spectra curves of Cyaniding-3-glucoside treated by PEF, the intensity of the peak at 333 nm increased as electric field and treatment time increased,while the intensity of the peak at 519 nm and the shoulder at 430 nm decreased, indicating Cyaniding-3-glucoside was degraded into chalcone.

关 键 词：高压脉冲电场 辅助提取 花色苷 矢车菊素-3-葡萄糖苷 降解作用 查尔酮 

分 类 号：TM835[电气工程—高电压与绝缘技术]