鲤鱼头中糖脂类活性物质的提取分离及抗肿瘤活性研究  

Isolation and Antitumor Activities of Glycolipids from Carp Head

作　　者：曹东旭[1,2] 杨书文[2] 刘娟娟[2] 王玥玮[2] 刘安军[1,2] 

机构地区：[1]天津市食品加工工程中心,天津300457 [2]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品科学》2009年第13期245-249,共5页Food Science

基　　金：天津科技大学引进人才科研启动基金项目(20060422)

摘　　要：以鲤鱼头为原料,研究其中糖脂类活性物质的提取、分离、鉴定及抗肿瘤活性。用乙醇提取粗脂,进一步用大孔吸附树脂HP-20、薄层色谱(TLC)及红外光谱对鱼头粗糖脂进行分离和初步鉴定。红外光谱分析结果初步表明,其为含有脂肪酸侧链的糖环类分子,符合糖脂的一般结构特征;细胞毒性检测(MTT法)糖脂的抗肿瘤活性结果表明:10～400μg/ml的鱼头糖脂作用24、48、72h后,能显著抑制人乳腺癌细胞MCF-7和人肝癌细胞Bel-7402的生长。苏木精-伊红(HE)染色结果表明,鱼头糖脂作用72h,MCF-7和Bel-7402细胞均产生明显的凋亡形态学变化。细胞周期分析结果表明,鱼头糖脂作用后,MCF-7和Bel-7402细胞被阻遏于细胞周期的S期,且这种S期积累具有明显的时间依赖性。Crude glycolipids extracted from ground fresh carp head with 90% ethanol were subjected to purification by HP 20 column chromatography and identification by thin layer chromatography （TLC） and infrared （IR） spectrum. And the antitumor activity of the glycolipids was determined by MTT assay for measuring cytotoxicity. The IR spectral analysis showed thin the molecules of the analyte contaied the carbohydrate rings with fatty acyl side chain as corresponding acid, which is a general structural feature of glycolipids. Incubation of glycolipids with hmnan breast cancer MCF-7 cells and liver canacer Bel-7402 cells for 24, 48 and 72 h significantly inhibited the growth of caner cells in vitro within the range of 10--400 μg/ml. The HE staining indicated that morphologicall alterations as indicative of aoptosis were observed in both MCF-7 cells and liver Bel-7402 when incubated with glycolipid extract for 72 h. Theur results suggested that, in breast and fiver cancer cells, glycoplipid from carp head delays cell cycle progression by promoting accumulation of cells in S phase without affecting the rate of apoptosis and this S-phase accumulation acts in a signigicant time-dependent manner.

关 键 词：鲤鱼头 糖脂 抗肿瘤活性 

分 类 号：Q538[生物学—生物化学]