啤酒废酵母自溶液中分离生物活性肽  

Separation of Active Polypeptides from Waste Beer Barm Autolysis

作　　者：王广莉[1] 凌秀梅[1] 胡鹏刚[1] 

机构地区：[1]贵州大学化学与化工学院蔡家关校区,贵州贵阳550003

出　　处：《酿酒科技》2009年第7期103-105,共3页Liquor-Making Science & Technology

摘　　要：对啤酒废酵母制取多肽的过程进行了研究。采用SephadexG-25凝胶层析分离出10种多肽,经过总抗氧化性的测定发现分离出来的多肽具有一定的总抗氧化性,其中组分E、F的总抗氧化性较强,E达到0.59 U/mg多肽,F达到0.58 U/mg多肽;洗脱曲线表明,自溶12 h能得到比较多的E、F组分。The preparation of active polypeptides from waste beer barm autolysis was studied. 10 kinds of peptides were separated by SephadexG-25. The activity of T-AOC of the polypeptides were determined with Iron （ Ⅱ ）- 1,10-Phenanthroline oxidation and the results showed that compositions E and F had strong T-AOC （E was 0.59 unit/mg peptide and F was 0.58 unit/mg peptide）. The elution curve suggested that 12 h autolysis of waste beer barm could obtain more E and F compositions.

关 键 词：啤酒 废酵母 自溶液 生物活性多肽 凝胶层析 

分 类 号：TS262.5[轻工技术与工程—发酵工程] TS261.4[轻工技术与工程—食品科学与工程]