黑曲霉AS3.4309产糖化酶条件的研究  

Glucoamylase production conditions of Aspergillus niger AS 3.4309

作　　者：周利南 国建娜[2] 蒋予箭[2] 励建荣[2] 

机构地区：[1]杭州酿造食品有限公司,浙江杭州310012 [2]浙江工商大学食品与生物工程学院,浙江杭州310035

出　　处：《中国酿造》2009年第7期53-57,共5页China Brewing

基　　金：2006年浙江省科技计划项目(2006C32013)

摘　　要：对黑曲霉AS3.4309液体曲产糖化酶的培养条件进行了研究。采用糖化力较强的黑曲霉进行液态培养,在单因素试验的基础上,采用4因素3水平正交试验对其产糖化酶的培养条件进行优化。结果表明,该菌株产糖化酶的最优培养基组成为:玉米粉15%,玉米浆7%,麸皮5%,豆粕粉2%,(NH4)2SO40.15%;培养条件为:接种量10%,发酵液起始pH4.5,30℃,200r/min,摇瓶培养7d,最高酶活力达到1294.52U/g。The liquid fermentation conditions for the glucoamylase production by Aspergillus niger AS 3.4309 were studied. Based on single factor test, the glucoamylase production conditions were optimized by L9 （3^4） orthogonal test. The results indicated that the optimal medium contained 15% corn starch, 7% com steep liquor, 5% bran, 2% soybean flour and 0.15% （NH4）2SO4. The culture conditions were as follows： inoculum size 10%, ini- tial pH 4.5, temperature 30℃, shaker speed 200 r/min and culture time 7 d. Under above conditions, the highest glucoamylase activity reached 1294.52 U/g.

关 键 词：黑曲霉 糖化酶 发酵 

分 类 号：TS201.3[轻工技术与工程—食品科学]