动物性食品中氯丙嗪残留的液相色谱法检测  

HPLC Determination of Chlorpromazine Residue in Animal-derived Foods

作　　者：洪月玲[1] 郝学飞[2] 董柯[1] 

机构地区：[1]郑州市疾病预防控制中心,河南郑州450053 [2]河南省农业科学院农业质量标准与检测技术研究中心,河南郑州450002

出　　处：《食品科学》2009年第14期269-271,共3页Food Science

基　　金：2006年河南省分析测试基金项目

摘　　要：建立动物性食品中氯丙嗪残留的HPLC检测法。试样用乙腈提取,经过碱性氧化铝柱纯化,采用反向C18色谱柱,二极管阵列检测器进行分析,以乙腈-0.1%磷酸溶液(7:3,V/V)为流动相,流速为1.0ml/min,检测波长为254nm,柱温40℃,外标法定量。方法最低检出限为4μg/kg,回收率为80%～101%。该方法样品处理简单,回收率高,灵敏度高,适合各种动物性食品中氯丙嗪残留的检测。A sensitive HPLC method was developed for the rapid determination of chlorpromazine residue in animal-derived foods. Samples were extracted with acetonitrile and cleaned up on a solid phase extraction column filled with alkaline alumina. Quantitative analyses were performed using the HPLC system equipped with a C18 column and a PDA detector. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid （7：3, V/V） at a flow rate of 1.0 ml/min, and the detection wavelength was set at 254 nrn. The limit of detection was 4 μg/kg and mean recoveries ranged from 80% to 101%. This method is accurate and feasible with simple pre-treatment for the detection of chlorpromazine residue in animal-derived foods.

关 键 词：动物性食品 氯丙嗪残留 高效液相色谱法(HPLC) 

分 类 号：TS207.5[轻工技术与工程—食品科学]