双水相体系提取SOD的研究  

Study on extraction of SOD in two-phase aqueous system

作　　者：李东旭[1] 吴蕾[1] 陈庆森[1] 刘晋生[2] 

机构地区：[1]天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津300134 [2]天津大学化工学院生物工程系,天津300072

出　　处：《食品工业科技》2009年第7期190-193,共4页Science and Technology of Food Industry

基　　金：教育部归国留学启动基金;天津市高等学校科技发展基金项目(重点ZD200716)

摘　　要：研究了聚乙二醇(PEG-4000)/磷酸钾双水相体系中,PEG浓度、磷酸钾浓度和pH对双水相萃取法从黄粉虫中提取超氧化物歧化酶(SOD)的影响。采用改良的邻苯三酚自氧化法测定SOD活性,并用SDS-聚丙烯酰胺凝胶电泳测定SOD的分子量。结果表明:双水相体系的质量组成为PEG12%,磷酸钾24%,pH7.1时,SOD的萃取效果最好,SOD纯化倍数达3.74,活性回收率达71.2%。The effects of concentration of polyethylene glycol, potassium phosphate and pH on extraction of superoxide dismutase （SOD） from the tenebrio molitor in aqueous two-phase system of PEG-4000 and potassium phosphate were studied. Superoxide dismutase activity was determined by improved pyrograllol autoxidation and the molecular weight of SOD was determined by SDS-polyacrylamide gel electrophoresis.The results showed that the optimum conditions were as follows： the mass composition two-phase aqueous system PEG 12%, potassium phosphate 24% ,pH7.1, SOD purification multiple could reach 3.74, the activity recovery rate could reach 7l.2%.

关 键 词：双水相系统 黄粉虫 超氧化物歧化酶 提纯倍数 

分 类 号：TS201.25[轻工技术与工程—食品科学]