鸭蛋黄渣中胆固醇的提取及GC-MS含量测定  

Extraction of cholesterol in duck egg yolk residue and GC-MS analysis

作　　者：马萍[1] 夏露[2] 马鹏飞[2] 王娜[1] 周敏[1] 

机构地区：[1]杭州市质量技术监督检测院,浙江杭州310019 [2]浙江大学动物科学学院,浙江杭州310029

出　　处：《食品工业科技》2009年第7期305-306,309,共3页Science and Technology of Food Industry

摘　　要：以已提取了卵磷脂的鸭蛋黄渣为原料，采用有机溶剂（丙酮）提取法提取胆固醇，设计了四因素三水平正交实验，结果表明：丙酮用量为鸭蛋黄渣质量的0．8倍，温度25℃，每次搅拌时间2h，提取次数5次的条件下，提取的胆固醇得率最高。采用GC—MS测定，胆固醇的纯度达到81．3％，此法的回收率为92．4％-96．3％，相对标准偏差1．22％，最低检出限0．02mg／kg。Cholesterol from duck egg yolk residue without lecithin is extracted by using organic solvent （acetone）. The experiment was designed according to four factors and three levels orthogonal table.The results showed that the optimum condition as follows：acetone volume was 0.8 times of the quality of egg yolk residue, temperature at 25~C,extraction 2h for each time, and extract 5 times.Determined by GC-MS,the content of cholesterol reached 81.3%.The recovery rate was 92.4%-96.3% ,the relative standard deviation was 1.22% ,The lowest detection limit was 0.02mg/kg.

关 键 词：胆固醇 丙酮 气质联用 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]