宁夏御马葡萄酒厂野生酵母菌株的分离筛选及分子鉴定  

Screening and molecular identification of wild yeasts isolated from Yuma vineyard of Ningxia

作　　者：王国平[1] 宋育阳[1] 裴颖芳[1] 刘延琳[1,2] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100

出　　处：《中国酿造》2009年第8期38-41,共4页China Brewing

基　　金：国家高技术研究发展计划"863计划"(2007AA102314);西北农林科技大学青年骨干支持计划项目(01140301)

摘　　要：利用26S rDNA D1/D2区序列分析法,对分离自宁夏御马葡萄园的22株野生酵母菌进行了鉴定,同时构建了22株供试菌株与相关模式菌株的系统发育树,分析了供试菌株与已知酵母菌的亲缘关系及其分类地位。结果表明:供试菌株被鉴定为5属7种,分别是酿酒酵母、巴氏酵母、葡萄汁有孢汉生酵母、克鲁维毕赤酵母、美极梅奇酵母和核果梅奇酵母。In this study ,22 yeast strains were collected from Yuma vineyard of Ningxia. These strains were identified by 26S rDNA D1/D2 domain sequence analysis. Phylogentic tree was constructed to study the relationship and confirmed the classification status of the yeast strains. The result showed that the selected yeast stains belonged to 7 species of 5 genera, namely, Saccharomyces cerevisiae, Saccharomyces pastorianus, Hanseniaspora uvarum, Pichia kluyver, Metschnikowia pulcherrima, Metschnikowia aft. fructicola and Candida zemplinina.

关 键 词：葡萄酒相关酵母菌 26S RDNA D1/D2区 序列分析 分类鉴定 

分 类 号：TS261.1[轻工技术与工程—发酵工程]