响应面法优化荞麦壳中原花青素的提取工艺  

Response Surface Optimization of Extraction Technology of Procyanidins from Buckwheat Shells

作　　者：查阳春[1] 杨义听[1] 胡晓菡[1] 李涛[1,2] 刘睿[1] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]湖北省产品质量监督检验研究院,湖北武汉430061

出　　处：《食品科学》2009年第16期189-192,共4页Food Science

基　　金：华中农业大学科技创新基金项目(2006-11);华中农业大学SRF项目(A06076)

摘　　要：为充分提取中国苦荞麦壳中的活性多酚,以70%乙醇-水为提取剂,通过二次旋转正交试验优化提取工艺,建立了提取得率的二次旋转回归方程,通过响应面分析及岭嵴分析得到了优化组合条件。结果表明,提取温度和时间对提取得率有显著影响(P<0.05),而料液比对提取得率的影响不显著,较优的提取工艺条件为提取温度73℃、提取时间118.9min、料液比1:16.9,其提取得率0.56%,理论预测值为0.59%,提取得率达到理论预测值的94.9%。Procyanidins in buckwheat shells was extracted with a mixture of 70% ethanol and water. In order to maximize the extraction of procyanidins, a rotatable orthogonal central composite design combined with response surface and ridge analyses was employed. Extraction temperature and time affected the yield of procyanidins significantly （P 〈 0.05）, while the effect of ratio of material to liquid was not significant. The optimum extraction conditions were as follows： ratio of material to liquid 1： 16.9, temperature 73 ℃, and extraction time 118.9 min. Under these conditions, the actual yield of procyanidins was 0.56%, which accounts for 94.9% of the theoretically predicted value of 0.59%.

关 键 词：荞麦壳 原花青素 提取工艺 旋转正交 

分 类 号：TS209[轻工技术与工程—食品科学]