淀粉接枝共聚物共固定糖化酶和葡萄糖异构酶研究  

Coimmobilization of Glucoamylase and Glucose Isomerase onto Starch Graft Copolymers Beads

作　　者：葛玉斌[1] 王树岩 孙铭一 王英明[1] 吴永革[1] 周慧[1] 李惟[1] 

机构地区：[1]吉林大学生命科学院,长春130023

出　　处：《吉林大学自然科学学报》1998年第3期99-102,共4页Acta Scientiarum Naturalium Universitatis Jilinensis

摘　　要：以多孔球状淀粉接枝共聚物为载体，以对一卜硫酸酯乙砜基苯胺（SESA）为载体活化剂，采用重氮化法共价偶联共固定糖化酶和葡萄糖异构酶．该共固定双酶体系适用于各种类型的淀粉底物，在PH6．0，60℃条件下，共固定双酶体系能将5％（质量浓度）DE－27糊精一步转化为果糖含量为20％的果葡糖浆，其催化效率是天然酶体系的1．6倍．Glucoamylase (GA) and glucose isomerase (GI), which are often used to produce high fructose syrup in industry, were co-immobilized onto 4-amino-benzene sulfonylethyl-porous starch graft copolymers beads by means of diazotization. The coimmobilized enzyme system showed a broad acceptance of various types of starch and dextrin substrates and a long-term (at least 8 h) linear rate of fructose production from DE-27 dextrin. The co-immobilized enzymes directly converted dextrin to glucose and fructose and increased fructose yield 0. 6-fold greater than the free enzymes.

关 键 词：糖化酶 葡萄糖异构酶 共固定化 淀粉接枝共聚物 

分 类 号：Q558[生物学—生物化学] TQ920[轻工技术与工程—发酵工程]