米根霉HZS6生产高光学纯度L-乳酸发酵条件的研究  

Study on Fermentation Conditions for High Optical Purity L-lactic Acid Produced by Rhizopus oryzae

作　　者：张晗星[1] 孟武[2] 封文涛[2] 李十中[1] 

机构地区：[1]清华大学核能与新能源研究院,北京100084 [2]山东轻工业学院,山东济南250353

出　　处：《山东农业科学》2009年第8期66-68,共3页Shandong Agricultural Sciences

基　　金：国家"十一五"科技支撑项目(2006BAD07A00)资助

摘　　要：通过研究和控制初始发酵条件,比较产物L-乳酸、D-乳酸组成变化,得出提高L-乳酸光学纯度的米根霉发酵生产条件:在pH 6.0、温度34℃、初始葡萄糖浓度100 g/L、NH4NO32 g/L、添加1.5 g/L L-乳酸的适合条件下,米根霉HZS6能够发酵生产光学纯度99%以上的L-乳酸,其转化率达到80%,终浓度为81.3 g/L。The effect of fermentation conditions on the optical purity of L - lactic acid produced by Rhizopus oryzae HZS6 was studied. Under the suitable conditions, that is to say, pH 6. 0, 34℃, 100 g/L initial glucose, 2 g/L NH4 NO3 and 1.5 g/L supplemented L - lactic acid, Rhizopus oryzae HZS6 could produce the L-lactic acid with the optical purity of 99%, the conversion rate of 80% and the final concentration of 81.3 g/L .

关 键 词：L-乳酸 D-乳酸 光学纯度 发酵调控 米根霉 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]