产D-塔格糖菌株的筛选与鉴定  

Screening and Identification of Two D-tagatose-producing Strains

作　　者：汪芙蓉[1] 徐虹[1] 李莎[1] 朱宏阳[1] 环民霞[1] 

机构地区：[1]南京工业大学食品与轻工学院,材料化学工程国家重点实验室,江苏南京210009

出　　处：《食品与发酵工业》2009年第8期15-19,共5页Food and Fermentation Industries

基　　金：国家“973”项目子课题(2007CB14304);江苏省省属高校自然科学重大基础专项(08KJA180001);国家自然科学基金(20674038);教育部科学技术重大研究项目(02056)

摘　　要：从土壤、泡菜汁、牛奶等样品中筛选获得2株高产D-塔格糖的菌株,根据16S rDNA序列比对并结合菌株的形态特征、生理生化特征分析,鉴定其分别为发酵乳杆菌和植物乳杆菌,且命名为Lactobacillus fermentumNXTag-1和Lactobacillus plantarum NXTag-2。菌株NXTag-1和NXTag-2表现出不同的产L-阿拉伯糖异构酶的能力,其最高酶活分别为5.93U/mL和5.05U/mL,以80g/LD-半乳糖为底物,分别产30g/L和26g/L的D-塔格糖。Two D-tagatose-producing strains, NXTag - 1 and NXTag - 2, were isolated from the samples of soil, pickle and milk. Based on the sequence analysis of 16S rDNA and a series of morphological and biochemical characteristics, NXTag - 1 was identified as Lactobacillus fermentum, NXTag -2 was identified as Lactobacillus plantarum. The maximum of L-arabinose isomerase activity of NXTag - 1 and NXTag - 2 reached 5.93 U/mL and 5.05 U/mL, the yield of D-tagatose were 30 g/L and 26 g/L （80 g/L D-galactose） , respectively.

关 键 词：乳酸菌 分离 鉴定 生物转化 D-塔格糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]