可见光分光光度法测定米曲霉发酵液中总黄酮  

Determination of total flavonoids in Aspergillus oryzae fermentation by visible spectrophotometry

作　　者：金红星[1] 胡炎华[1] 成文玉[1] 

机构地区：[1]河北工业大学化工学院,天津300130

出　　处：《中国酿造》2009年第9期142-144,共3页China Brewing

摘　　要：米曲霉可发酵生产黄酮类物质,为确定发酵液中总黄酮含量,采用可见光分光光度法(A510)进行测定,并优化了测定条件。确定乙醇浓度为95%(体积分数),亚硝酸钠浓度为4%(质量分数),硝酸铝浓度为10%(质量分数)以及氢氧化钠浓度为4%(质量分数)时最适用于米曲霉发酵液中总黄酮含量的测定。最后分别用芦丁和槲皮素物质的量为标准确定总黄酮含量。The concentration of flavonoids in Aspergillus oryzae fermentation was determined by visible spectrophotometry （A510）. The optimal determination conditions were obtained as follows： ethanol concentration 95%, sodium nitrite concentration, 4%, aluminum nitrate concentration 10% and sodium hydroxide concentration 4%. The total flavonoids content were assayed using rutin and quercetin as standard.

关 键 词：可见光分光光度法 米曲霉 发酵液 总黄酮 

分 类 号：O657.32[理学—分析化学]