基因芯片在黄曲霉毒素生物合成相关基因差异表达分析中的应用研究  

Application of Gene Chips in Analysis of Differential Expressions of Genes Involving in Aflatoxin Biosynthesis

作　　者：胡娜[1] 许杨[2] 

机构地区：[1]江西科技师范学院生命科学学院,江西南昌330013 [2]南昌大学中德联合研究院,江西南昌330047

出　　处：《食品科学》2009年第17期208-211,共4页Food Science

基　　金：江西科技师范学院青年创新基金项目

摘　　要：为寻找与黄曲霉毒素生物合成密切相关的基因,本研究采用诱导产毒培养的方法培养寄生曲霉AS3.4407,获得产毒与不产毒两种性状的菌丝体。应用基因芯片技术和RT-PCR方法筛选与黄曲霉毒素生物合成相关的差异表达基因。研究结果表明产毒菌丝与不产毒菌丝中与产毒相关的部分基因存在差异表达。芯片扫描的结果与RT-PCR的扩增结果一致,均显示有6个基因存在表达差异,它们分别是aflA、aflE、aflF、aflR、aflT和aflX。本研究证实aflR基因表达的改变与黄曲霉毒素的产生密切相关。The aflatoxin （AF） production was induced inAspergillusparasiticus AS3.4407 by shifting the cultures from nonconducive to conducive medium and aflatoxin Bt （AFBl）-producing and non-AFBl-producing mycelia were obtained and lyophilized. Subsequently, differential expressions of genes involving in the AF biosynthesis were screened using gene chips and RT-PCR. The results from gene chips were consistent with those from RT-PCR amplification, suggesting differential expressions of six genes including aflA, aflE, aflF, aflR, aflT and aflX. This study proves that the differential expression of aflR is closely related to the aflatoxin production.

关 键 词：寄生曲霉 黄曲霉毒素 基因芯片 差异表达基因 

分 类 号：Q789[生物学—分子生物学]