Increase in sphingolipid catabolic enzyme activity during aging  

作　　者：Santosh J SACKET Hae-young CHUNG Fumikazu OKAJIMA Dong-soon IM 

机构地区：[1]Laboratories of pharmacology and , Pusan National University, Busan 609-735, Republic of Korea [2]Biochemistry, College of Pharmacy (BK21 Project) and Longevity Institute of Life Science and Technology , Pusan National University, Busan 609-735, Republic of Korea [3]Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371, Japan

出　　处：《Acta Pharmacologica Sinica》2009年第10期1454-1461,共8页中国药理学报（英文版）

摘　　要：Aim： To understand the contribution of sphingolipid metabolism and its metabolites to development and aging. Methods： A systemic analysis on the changes in activity of sphingolipid metabolic enzymes in kidney, liver and brain tissues during development and aging was conducted. The study was conducted using tissues from 1-day-old to 720-day-old rats. Results： Catabolic enzyme activities as well as the level of sphingomyelinase （SMase） and ceramidase （CDase） were higher than that of anabolic enzyme activities, sphingomyelin synthase and ceramide synthase. This suggested an accumulation of ceramide and sphingosine during development and aging. The liver showed the highest neutral-SMase activity among the tested enzymes while the kidney and brain exhibited higher neutral-SMase and ceramidase activities, indicating a high production of ceramide in liver and ceramide/sphingosine in the kidney and brain. The activities of sphingolipid metabolic enzymes were significantly elevated in all tested tissues during development and aging, although the onset of significant increase in activity varied on the tissue and enzyme type. During aging, 18 out of 21 enzyme activities were further increased on day 720 compared to day 180. Conclusion： Differential increases in sphingolipid metabolic enzyme activities suggest that sphingolipids including ceramide and sphingosine might play important and dynamic roles in proliferation, differentiation and apoptosis during development and aging.Aim： To understand the contribution of sphingolipid metabolism and its metabolites to development and aging. Methods： A systemic analysis on the changes in activity of sphingolipid metabolic enzymes in kidney, liver and brain tissues during development and aging was conducted. The study was conducted using tissues from 1-day-old to 720-day-old rats. Results： Catabolic enzyme activities as well as the level of sphingomyelinase （SMase） and ceramidase （CDase） were higher than that of anabolic enzyme activities, sphingomyelin synthase and ceramide synthase. This suggested an accumulation of ceramide and sphingosine during development and aging. The liver showed the highest neutral-SMase activity among the tested enzymes while the kidney and brain exhibited higher neutral-SMase and ceramidase activities, indicating a high production of ceramide in liver and ceramide/sphingosine in the kidney and brain. The activities of sphingolipid metabolic enzymes were significantly elevated in all tested tissues during development and aging, although the onset of significant increase in activity varied on the tissue and enzyme type. During aging, 18 out of 21 enzyme activities were further increased on day 720 compared to day 180. Conclusion： Differential increases in sphingolipid metabolic enzyme activities suggest that sphingolipids including ceramide and sphingosine might play important and dynamic roles in proliferation, differentiation and apoptosis during development and aging.

关 键 词：AGING SPHINGOLIPID METABOLISM SPHINGOMYELINASE CERAMIDASE sphingomyelin synthase ceramide synthase 

分 类 号：Q545.03[生物学—生物化学] TS201.7[轻工技术与工程—食品科学]