高效液相色谱—蒸发光散射检测法同时测定单糖、双糖及低聚果糖  

Simultaneous HPLC-ELSD Determination of Monosaccharide,Disaccharide and Fructo-oligosaccharides

作　　者：张媛媛[1] 聂少平[1] 万成[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2009年第18期237-239,共3页Food Science

基　　金：教育部"长江学者和创新团队发展计划"项目(IRT0540);食品科学与技术国家重点实验室目标导向资助项目(SKLF-MB-200806)

摘　　要：建立一种高效液相色谱-蒸发光散射检测(HPLC-ELSD)同时对葡萄糖、果糖、蔗糖、蔗果三糖、蔗果四糖和蔗果五糖进行测定的方法。采用氨基柱分离,乙腈-水为流动相,梯度洗脱,流速1.0ml/min,ELSD漂移管温度85℃,载气为空气,流速2.0L/min。结果表明,在该条件下六种糖的组分都能够得到较好的分离,线性良好,回收率为97.62%～99.55%,相对标准偏差(RSD)小于3%(n=5)。该方法稳定、准确,适合于单糖、双单及低聚糖的检测及含量测定。A HPLC-ELSD method was established for the simultaneous quantitative analysis of glucose, fructose, sucrose, GF2, GF3, and GF4. The chromatographic separation was achieved on an amino column using a mobile phase composed of a mixture of acetonitrile and water at a flow rate of 1.0 ml/min by gradient elution. The ELSD drift tube temperature was set at 85 ℃ and the carrying gas was air. Under the above conditions, all glucose, fructose, sucrose, GF2, GF3 and GF4 could be well separated, and the spiked recoveries were 97.62% - 99.55% with a RSD less than 3% （n = 5）. This method is accurate, rapid, simple and reproducible, and thus it is suitable for the determination of monosaccharides, disaccharides, and oligosaccharides.

关 键 词：高效液相色谱-蒸发光散射检测 单糖 双糖 低聚果糖 

分 类 号：TS207.3[轻工技术与工程—食品科学]