棒曲霉糖化酶的初步研究  

Preliminary Studies on Glucoamylase of Aspergillus clavatus

作　　者：廖美德[1] 

机构地区：[1]湖北农学院农业工程系

出　　处：《湖北农学院学报》1998年第3期250-252,共3页Journal of Hubei Agricultural College

摘　　要：棒曲霉（ＡｓｐｅｒｇｉｌｕｓｃｌａｖａｔｕｓＤｅｓ）ＨＤ－１１是从绿衣观音土曲中分离得到的，具有较高糖化酶活性的菌株。初步研究结果表明，３０℃下，棒曲霉麸曲培养３０～４０ｈ，其产酶量达到高峰。糖化酶最适作用温度５０～６０℃，最适作用ｐＨ３．０～４．０。糖化酶在４５℃以下，ｐＨ４０～８．０范围内稳定，对淀粉水解程度为７９％，产物为葡萄糖、麦芽糖和少量低聚糖。spergillus clavatus strain HD-11 was isolated from the Green Kaolinite Koji. When cultured at 30 ℃ for 30 ～40 h in bran medium, its glucoamylase activity got the peak. The optimum pH and temperature for the enzyme were 3～4 and 50～60 ℃ respectively. The enzyme was stable at the temperature below 45 ℃ and the pH varying from 4 to 8. The enzyme can hydrolyse starch into glucose, maltose and small amount of oligosaccharide.

关 键 词：棒曲霉 糖化酶 酒曲 

分 类 号：Q949.327.1[生物学—植物学] TS261.1[轻工技术与工程—发酵工程]