高效液相色谱法测定血清叶黄素、番茄红素和β-胡萝卜素  

DETERMINATION OF LUTEIN,LYCOPENE,AND β-CAROTENE IN SERUM BY HPLC

作　　者：章清[1] 沈新南[1] 刘珊林[2] 

机构地区：[1]复旦大学公共卫生学院营养与食品卫生学教研室,公共卫生安全教育部重点实验室 [2]复旦大学上海医学院,上海200032

出　　处：《营养学报》2009年第5期498-501,共4页Acta Nutrimenta Sinica

摘　　要：目的建立血清中叶黄素、β-胡萝卜素、番茄红素的高效液相色谱测定法。方法Inertsil ODS C18色谱柱（250×4.6mm,粒径5μm）;选用甲醇-乙腈-四氢呋喃（60：20：20）,含0.01%的抗坏血酸为流动相;流速为1.0ml/min;检测波长为450nm。结果叶黄素、番茄红素和β-胡萝卜素的保留时间分别为4.3min、12.4min和17.0min,回收率分别为97.98%～102.80%、97.24%～101.76%、98.56%-100.4%,日内和日间RSD分别为2.26%和3.02%、4.34%和5.02%、1.81%和2.52%。标准曲线的线性范围为0.05～2.00μg/ml。90名正常人血清中叶黄素、番茄红素与β-胡萝卜素的含量分别为0.34±0.17μg/ml、0.28±0.16μg/ml、0.49±0.25μg/ml。结论HPLC法测定人血清叶黄素、番茄红素和β-胡萝卜素浓度方法可靠,样品处理快速简便。Objective To establish the HPLC method for accurate and precise measurement of the major carotenoids in plasma using HPLC detection. Method The chromatographic analysis was performed using an analytical scale C18 reversed phase column （250 mm×4.6 mm i.d.） The mobile phase for chromatography was methanol：acetonitrile： tetrahydrofuran （60：20：20） containing 0.01% ascorbic acid. The flow rate was set at 1 ml/min. Chromatographic runs were monitored at 450nm. Results The average retention time for lutein, lycopene and β-carotene were 4.3 min, 12.4 min and 17.0 min respsetivuly. The recovery rate was as follows：lutein 97.98%- 102.80%, lycopene 97.24%- 101.76%, β-carotene 98.56%- 100.4%. The intra-day and inter-day RSD were 2.26% and 3.02%, 4.34% and 5.02%, 1.81% and 2.52% for lutein, lycopene, and β-carotene respectively. Ninety healthy inhabitants were enrolled in this study. Mean plasma concentrations of lutein, lycopene and β-carotene were 0.34±0.17 μg/ml, 0.28±0.16 μg/ml, 0.49±0.25μg/ml. Conclusion This method was simple, rapid and accurate for determination of lutein, lycopene and β-carotene in serum.

关 键 词：高效液相色谱 叶黄素 番茄红素 Β-胡萝卜素 血清 

分 类 号：R151.3[医药卫生—营养与食品卫生学]