纤维素降解的菌株筛选及其运用  

Screening of Cellulose Degradation Strains and their Application

作　　者：蒋加拉[1] 刘素纯[1] 关美玲[1] 

机构地区：[1]湖南农业大学食品科技学院,湖南长沙410128

出　　处：《现代生物医学进展》2009年第18期3451-3454,共4页Progress in Modern Biomedicine

基　　金：湖南农业大学人才科学基金项目

摘　　要：目的:筛选降解稻草纤维素菌株,为纤维素的高效降解提供理论依据。方法:采用羧甲基纤维素钠刚果红培养基与滤纸条培养基从采集的腐木、腐土和腐叶等样品中筛选出纤维素降解菌。然后经液态发酵后测定其羧甲基纤维素酶活力与降解稻草的天然纤维素酶活力,综合考虑这两种酶活力,对其进行单独与混合发酵培养。筛选分解稻草能力较强的菌株组合。结果:初筛到5株真菌和5株细菌纤维素降解力较优的菌株。经酶活力测定后,得到分解纤维素能力较强的两株真菌F3和F5与两株细菌B1和B5,其中F3和B1的羧甲基纤维素酶活分别为705.6U、214.6U;F5和B5天然纤维素酶活分别为466.5U、204.8U。混合培养在一定程度上能提高纤维素酶活,F3/F5具有稳定而较高的酶活力,某时间段酶活高达646.8U,且后续酶活力也保持在较高水平。而F3/B5在某时间段的酶活高达788.6U。结论:菌株的混合培养可以提高纤维素酶活。Objective： The aim of the research was to supply the oretical basis for high efficient decomposition of cellulose of rice straw. Method： The strains that could decompose cellulose better were screened out from rotten wood, mulch and rotten leaves, by sodium carboxymethyl cellulose and filter paper medium. The strains which have strong cellulase activity by comprehensive considering sodium carboxymethyl-cellulose（CMC） enzyme activity and natural cellulase activity of degrading rice straw by liquid fermenting. Then they were cultured solely and by mixed fermentation. Rusults： 5 strains of fungi and 5 strain of bacteria were obtained preliminarily, fungi （F3.F5）and bacterium（ B1,B5 ）were examined to have more strong cellulase activity. The CMC enzyme activity of F3,Bl is up to 705.6U, 214.6 U respectively, and the natural cellulase activity of F5,B5 attain to 466.5 U,204.8 U respectively. Cellulose activity of strains in the mixed culture were improved to some degree. The enzyme activity of F3/F5 was stable and upper to 646.8U at some time and the subse- quent activity could also maintain at a high level. F3/B5 showed strong enzyme activity at some time get to 788.6U.Conelusion： The mixed culture of strains could increase cellulose activity.

关 键 词：纤维素酶 降解 筛选 酶活力 

分 类 号：Q946[生物学—植物学]