转ChIFN-γ基因烟草抗虫作用初步研究  

A preliminary study on the insect-resistant role of transgenic ChIFN-γ tobacco

作　　者：吴拥军[1,2,3] 赵德刚[1,2] 许文钊[1] 李耀中[1] 詹寿年[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省农业生物工程重点实验室,贵州贵阳550025 [3]教育部绿色农药与农业生物工程重点实验室,贵州贵阳550025

出　　处：《云南大学学报（自然科学版）》2009年第6期629-631,643,F0002,F0003,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家科技支撑计划资助项目(2007BAD59B06);中国国际合作资助项目(2007DFA31260)

摘　　要：对田间烟蚜的自然发生情况观察和抗虫试验的研究显示,转ChIFN-γ基因烟叶具有显著的抗虫效果.为探索转基因烟草的抗虫机理,测定了叶片中烟碱含量并对植株组织进行切片染色.结果表明,烟碱含量与对照无显著差异;转基因烟草叶片增厚,细胞致密,细胞壁增厚可能是导致抗蚜虫、抗斜纹夜蛾的最直接因素之一.The study of the natural infection of aphid and the insect-resistant assay of transgenic ChIFN-γ tobacco in field verified that transgenic tobacco demonstrated a significant enhancement in insect resistance.To reveal the mechanism of the insect resistance to the transgenic ChIFN-γ tobacco,the content of nicotine in the transgenic leaves was determined and the tissue stained technique was carried out.No significant difference was obtained in the contents of nicotine between the transgene and wild type.However,the leaf thickness,cell density,cell wall thickness of the transgenic tobacco showed an improved trend in the transgenes,which might suggest that these parameters probably contributed to the positive resistance to tobacco aphids and Spodoptera litura larvae.

关 键 词：ChIFN-γ基因 烟草 烟碱 组织切片 抗虫机制 

分 类 号：Q949.99[生物学—植物学]