化学诱变选育适量高产SO_2啤酒酵母菌株  

Screening of Saccharomyces cerevisiae with high yield of SO_2 by chemical mutation

作　　者：王勇[1] 程殿林[1] 尹明浩[1] 王亚楠[1] 

机构地区：[1]青岛大学生物系,山东青岛266071

出　　处：《中国酿造》2009年第11期13-16,共4页China Brewing

基　　金：山东省自然科学基金(Y2006D05);山东省教育厅科技项目(J07YJ19-1)

摘　　要：采用硫酸二乙酯对啤酒酵母出发菌株SY-8进行化学诱变,通过不同硫源鉴别培养基的反复筛选,得到适量高产SO2的菌株MS-10。发酵实验结果显示MS-10的SO2生成量在25mg/L左右,与原始出发菌株相比有很大提高,其遗传物质经RAPD分析,与出发菌株SY-8相比也有较大差异,是1株适量高产SO2的新菌株。S. cerevisiae strain SY-8 was treated with DES （diethyl sulfate） for chemical mutatin. Strain MS-10 was obtained after screening with medium contained different sulfur sources. Yield of SO2 by MS-10 was about 25 mg/L, which is much more than strated SY-8. MS-10 is a new strain with high capacity of SO2 yield. MS-10 has significantly different genetic sequences from SY-8 analyzed with RAPD.

关 键 词：啤酒酵母 诱变 二氧化硫 

分 类 号：Q93-331[生物学—微生物学]