响应面分析法优化牛蒡胡萝卜素的提取工艺  

Optimization for carotene extraction from the roots of Arctium lappa L. by response surface method

作　　者：张彧[1] 聂艳玲[1] 张晓娟[1] 王营[2] 葛丽丽[2] 潘肇仪[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]沈阳红梅企业集团有限责任公司,辽宁沈阳110026

出　　处：《大连工业大学学报》2009年第6期397-400,共4页Journal of Dalian Polytechnic University

摘　　要：采用响应面分析法对牛蒡根中胡萝卜素的提取工艺进行优化,在单因素试验的基础上选取试验因素与水平,根据正交旋转组合试验设计原理采用三因素五水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以牛蒡根胡萝卜素提取率为响应值做响应面和等高线图,在分析各个因素的显著性和交互作用后得出牛蒡根胡萝卜素微波辅助有机溶剂浸提的最佳工艺条件:功率70 kW,提取溶剂为丙酮-石油醚(体积比为1∶2),牛蒡粉粒度为60目,提取2次,温度49.2℃,时间41.8 min,液固比22.8 mL/g,该条件下得到的最大提取率为5.073μg/g。Optimum conditions for the extraction of B-carotene from the roots of Arctiurn lappa L. were studied by the orthogonal rotation combination experiment design and response surface method on the basis of single factor experiments. The extraction rate of β-carotene from the roots of Arctium lappa L. could be up to 5. 073 μg/g when the extraction was carried by organic solvent assisted by ultrasonication at the conditions V（AC） ： V（PE） = 1 ： 2 and 70 kW, the particle size of burdock is 60, the ratio of solvent to material is 22.8 mL/g, 41.8 min, 49.2 ℃ and extracted twice.

关 键 词：牛蒡根 胡萝卜素 响应面法 

分 类 号：TS201.2[轻工技术与工程—食品科学]